Remel bactidrop lactophenol aniline blue is for staining fungal elements. Revisions highlighted packing group classification and labelling have been performed according to eu. Place a drop of seventy percent alcohol on a clean microscope slide. Lactophenol cotton blue solution, readily available from biological supply houses, stain fungi blue only living tissue picks it up. What safe alternatives are there to lactophenol cotton blue for fungal. Lactophenol cotton blue, fungal stain, 15ml, by hardy diagnostics. Stain fungal elements with thermo scientific remel lactophenol aniline blue. Lactophenol cotton blue stain fungal element stain for.
Manual of clinical microbiology, american society for microbiology, washington, d. Place suspected fungi on a glass slide, drop lactophenol cotton blue solution on it, cover it with a cover slip and examine with an optical microscope. Lactophenol blue solution is readytouse staining solution for fungi staining in bacteriology with samples of human origin. What safe alternatives are there to lactophenol cotton. Lactophenol cotton blue used for wet mounts in the examination of yeasts and molds, serving as mounting fluid and stain. Lactophenol cotton blue is used as staining solution for fungi. Lactophenol blue solution for microscopy, for staining molds. Product should not be used if there are any signs of contamination. Lactophenol cotton blue stain definition of lactophenol. Lactophenol anilin blue solution, lactophenol cotton blue solution 635. Central drug house p ltd 728 vardaan house new delhi2 india. Lactophenol aniline blue may be adequate for presumptive identification, however, colonial morphology and further microscopic examination may be necessary for definitive identification.
Lactophenol blue solution, for microscopy, for staining molds milliporesigma synonyms. State the scientific name of the yeast studied in the laboratory you will use yeast over and over in this lab so know this cold for exams and quizzes. We generally do not inject more than 15 ml of 7% phenol in a session. This readytouse solution is especially for airdried, heatfixed smears of bacteriological material, e. Z7 the hardy diagnostics manufacturing facility and quality management system comply with the fdas good manufacturing practices gmp and have achieved iso 485 certification as a medical device. Lactophenol blue solution is a mounting medium and staining agent used in the. To perform tests, squeeze the dropper to crush the ampule.
Bd diagnostic 261188 dropper lactophenol stain, cotton blue pack of 50. Slide culture technique and lactophenol cotton blue. Jul 20, 2014 in this prospective study, which was carried out from march to september, 20, we have evaluated the performance of iodineglycerol in comparison to lactophenol cotton blue lpcb and congored formaldehyde as an alternative method for identification of filamentous fungi. Slide culture technique and lactophenol cotton blue staining. Oct 18, 2016 procedure a drop of lactophenol cotton blue was placed on a clean glass slide. The lactophenol cotton blue lpcb wet mount is most widely used method for the staining and observation of fungi. This products resides on a fisher scientific gsa or va contract. Our lactophenol blue stain is used as a mounting medium.
Immediately flush eyes with gentle but large stream of water for at least 15 minutes, lifting lower and upper eyelids occasionally. If you are viewing this page as a nonregistered user, the prices displayed is list price. Pdf evaluation of lactophenol cotton blue for wet mount. This item is sold and shipped to schools and businesses only. Lactophenol cotton blue lcb is a mixture of methyl blue, a histological stain, and lactophenol it is used in wetmount preparations for visualization of fungal. Organisms suspended in the stain are killed due to the presence of phenol. For pigmented strains of moulds, lactophenol without cotton blue is available and can be used quality control internal quality control of the lactophenol blue solution must be performed regularly on known reference organisms to ensure the performance of the mounting solution. Cotton blue lactophenol page 1 of 8 material safety data sheet according to 91155ec section 1. Place the coverslip on the drop avoiding air bubbles to be trapped. Cotton blue is an acid dye that stains the chitin present. Place suspected fungi on a glass slide, drop lactophenolcotton blue solution on it, cover it with a cover slip and examine with an optical microscope. Add one or two drops of lactophenol cotton blue stain before alcohol gets off.
Relevant identified uses of the substance or mixture and uses advised against identified uses laboratory reagent. Lactophenol, staining solutions, by loba chemie, india. The slides were analysed, and the images were captured with a leica dc 300f digital camera attached to a leica dmld microscope. Supplied in crushable ampules enclosed in a protective plastic sheath with a convenient dropper dispenser tip. Product name lactophenol blue solution sigmaaldrich. Safety data sheet bactidroptm lactophenol aniline blue. Jorvet j326b lactophenol cotton blue stain for animal use. Hardy diagnostics lactophenol cotton blue stain is recommended for mounting. Bactidroptm lactophenol aniline blue safety data sheet date of issue 27092007. A small part of fungal colony was taken from potato dextrose agar pda plate using a sterile needle and it was placed on the drop of lactophenol cotton blue that had been taken on the glass slide.
Staining in 5% blue cotton in lactophenol for 20 min macedo, 1997. Lactophenol blue solution stain fungal, sigmaaldrich vwr. Lactophenol cotton blue is used for staining solution for fungi. Comparison of microscopic morphology of fungi using. Manual of clinical mycology prepared under the auspices of the division of medical. Dermatophyte test medium an overview sciencedirect topics. Dissolve the cotton blue in distilled water and leave overnight to eliminate insoluble dye. State the scientific name of the yeast studied in the laboratory you will use yeast over and over in. What is the role of phenol in lactophenol cotton blue. The yeast with lactophenol cotton blue is called a simple stain because it stains the yeast cells, so they can be more readily observed under the microscope. Milliporesigma lactophenol blue solution lactophenol blue.
Lactophenol cotton blue solution mdl no mfcd00146574 density. The high concentration of the phenol deactivates lytic cellular enzymes thus the cells do not lyse. Material safety data sheet section 1 product and company information product name. Bbl lactophenol cotton blue stain droppers are used in wet amounts for examination of fungi 50sp, 500ca. New staining technique for fungalinfected plant tissues. In this prospective study, which was carried out from march to september, 20, we have evaluated the performance of iodineglycerol in comparison to lactophenol cotton blue lpcb and congored formaldehyde as an alternative method. Methyl blue is also available in mixture with water blue, under name aniline blue ws, aniline blue, china blue, or soluble blue. The material is stained in one step with lactophenol blue solution.
Lpcb100, lpcb500, lpcba100, lpcba500 recommended use of the chemical and restriction on use. Lactophenol picric acid s017 intended use lactophenol picric acid is used as fungal staining solution. Ten milliliters of a 7% solution of phenol contains 0. A drop of lactophenol solution is placed onto a clean glass microscope slide. Page 17 safety data sheet according to whs regulations printing date 29. The lethal range of phenol that gets into the central nervous system is approximately 8. A small part of fungal colony was taken from potato dextrose agar pda plate using a sterile needle and it was placed on the drop of lactophenol cotton blue that had been taken on. Stain airdried, heatfixed smears of bacteriological material, e. Dropper contains oneday supply of reagent or stain, hermetically sealed inside an ampule. Lpcb is a stain used for making semipermanent microscopic preparation of fungi. Details of the supplier of the safety data sheet supplier prolab diagnostics 3. Search results for lactophenol cotton blue at sigmaaldrich. I read some of journal and they used lactophenol cotton blue stain but its hard to find it here. Diagnosis of dermatophytoses is based on the characteristic gross appearance of the lesions, cytologic examination of hairs or skin scrapings treated with potassium hydroxide examined with contrast microscopy or stained with lactophenol cotton blue, histologic examination of skin biopsy table 2.
Hermetically sealed in ampule oneday supply dropbydrop dispensing directions to perform tests, squeeze the dropper to crush the ampule. Procedure a drop of lactophenol cotton blue was placed on a clean glass slide. If a phenol block seems ineffective after several weeks, it may be repeated. Lactophenol aniline blue intended use remel lactophenol aniline blue is a stain recommended for use in wet mount preparations for microscopic examination of fungi. Staining techniques in microbiology linkedin slideshare. Lactophenol cotton blue wet mount is simple and widelthe y used staining method for fungi. Lactophenol cotton blue stain fungal element stain. Proslide microscope slides, frosted, premium, 3x1 inches x 1mm, 72 per box, by hardy diagnostics. Add a drop of lactophenol cotton blue solution on a slide. Lactophenol cotton blue solution material safety data. The lactophenol cotton blue lpcb wet mount preparation is the most widely used method of staining and observing fungi and is simple to prepare. Lactophenol cotton blue lpcb mounts of corneal ulcer scrap ings were. Lactophenol cotton blue stain other means of identification.
Preparation of lactophenol cotton blue lpcb slide mounts. Summary and explanation linder recommended lactophenol as a mounting fluid for examination and preservation of fungi. Jorvet j326b lactophenol cotton blue stain indications. Composition ingredients formula adjusted, standardized to suit performance parameters directions principle and interpretation fungus are eukaryotic organism and they are classified into two main groups that is yeast and molds. Lactophenol helps preserve the fungal structures and kill any microorganisms. A solution of phenol and lactic acid in glycerol and water. What safe alternatives are there to lactophenol cotton blue. Lactophenol cotton blue stain fungal element stain for mycology.
Examine under microscope using 10x and 40x objective. Lactophenol cotton blue stain is formulated with lactophenol, which serves as a mounting fluid, and cotton blue. Mix the specimen whether a skin scraping, fluid exudate or tissue with two drops of the 10% koh on a clean slide. Preparation of lactophenol cotton blue slide mounts. Bd diagnostic 261188 dropper lactophenol stain, cotton. Sigmaaldrich 3050 spruce street saint louis mo 63103 usa telephone. Invert the dropper for convenient dropbydrop dispensing.
Other information msds first issued 28022007 msds data revised 27092007 uses stain for fungal elements. Product name lactophenol cotton blue product number pl. Properties, disease and laboratory diagnosis april 27, 20 1. Prepare lactophenol cotton blue wet mount of fungal colony. Lactophenol cotton blue lpcb for the staining and microscopic identification of fungi. Lactophenol cotton blue solution is a mounting medium and staining agent used in the preparation of slides for microscopic examination of fungi. On the first day, dissolve the cotton blue in the distilled water. The lpcb also serves as a mounting medium for placing on a cover slip. Lactophenol cotton blue lpcb wet mount preparation it is used to demonstrate fungi and fungal elements under the microscope in lpcb wet mount preparation. Bd diagnostic 261188 dropper lactophenol stain, cotton blue. Wet mount preparation is the most widely used method of staining and observing fungi. Some article used methylen blue stain but i dont know about the concentration that i should use. Identification of the substancemixture and of the companyundertaking 1. This readytouse solution is especially for airdried, heatfixed smears of bacteriological material.
The fungi identified by characteristic microscopic morphology such as shape, size, arrangements of spores and hyphae. Jan 11, 2020 the lactophenol cotton blue lpcb wet mount is most widely used method for the staining and observation of fungi. Lactophenolcotton blue solution, readily available from biological supply houses, stain fungi blue only living tissue picks it up. Check for and remove contact lenses if present and easy to do. Consult appropriate references for further instructions. Lactophenol blue solution lactophenol cotton blue solution. Composition, preparation and uses september 10, 2016 3. Search results for lactophenol blue solution at sigma aldrich. Pdf the use of lactophenol cotton blue lpcb stain for wet mount. Lactophenol cotton blue solution is a mounting medium and staining agent used in the preparation of slides for microscopic examination of. Lactophenol anilin blue solution, lactophenol cotton blue solution.
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